Nature Search

Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons

An important goal for neuroscience tool development is improving the performance of genetically encoded voltage sensors. Here, the authors report two mutants of Arch, ‘Archers’, with high baseline fluorescence and sensitivity and show proof of principle for detecting voltage changes in response to sensory stimulus in live C. elegans.

Nicholas C. Flytzanis
Claire N. Bedbrook
Viviana Gradinaru
Research15 Sept 2014
Nature Communications

Volume: 5, P: 1-9
Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain

Crossing the blood–brain barrier in primates is a major obstacle to gene delivery in the brain. Here an adeno-associated virus variant (AAV.CAP-Mac) is identified and demonstrated for crossing the blood–brain barrier and delivering gene sequences to the brain of different non-human primates species.

Miguel R. Chuapoco
Nicholas C. Flytzanis
Viviana Gradinaru
ResearchOpen Access10 Jul 2023
Nature Nanotechnology

Volume: 18, P: 1241-1251
AAV capsid variants with brain-wide transgene expression and decreased liver targeting after intravenous delivery in mouse and marmoset

The authors developed AAV capsids for robust transgene expression in the brain with decreased liver targeting after non-invasive administration in mice and marmosets, enabling more targeted systemic gene delivery to the brain.

David Goertsen
Nicholas C. Flytzanis
Viviana Gradinaru
Research09 Dec 2021
Nature Neuroscience

Volume: 25, P: 106-115
Fluorescent boost for voltage sensors

The development of a voltage sensor in which a microbial rhodopsin protein is fused with a fluorescent protein enables the neuronal activity of single cells in live animals to be measured with unprecedented speed and accuracy.

Viviana Gradinaru
Nicholas C. Flytzanis
News & Views27 Jan 2016
Nature

Volume: 529, P: 469-470
Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation

Adeno-associated viruses (AAVs) can be targeted in a tissue-specific manner, but their tissue accumulation cannot be assessed in a non-invasive manner. Here the authors conjugate a multivalent chelator labelled with Cu-64 to the surface of AAVs and image the brain accumulation of the PHB.eB capsid by PET.

Jai Woong Seo
Elizabeth S. Ingham
Katherine W. Ferrara
ResearchOpen Access30 Apr 2020
Nature Communications

Volume: 11, P: 1-13
Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

This protocol describes how to fix, embed, clear and stain excised organs or whole organisms to create optically transparent samples. This versatile protocol is able to process a wide range of sample types for high-resolution imaging.

Jennifer B Treweek
Ken Y Chan
Viviana Gradinaru
Protocols22 Oct 2015
Nature Protocols

Volume: 10, P: 1860-1896

Search

Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons

Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain

AAV capsid variants with brain-wide transgene expression and decreased liver targeting after intravenous delivery in mouse and marmoset

Fluorescent boost for voltage sensors

Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation

Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

Search

Quick links

Search

Filter By:

Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons

Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain

AAV capsid variants with brain-wide transgene expression and decreased liver targeting after intravenous delivery in mouse and marmoset

Fluorescent boost for voltage sensors

Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation

Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping

Search

Quick links