This protocol details the quantitative imaging of apoplastic pH in Arabidopsis thaliana roots using confocal microscopy combined with the fluorescent pH probe 8-hydroxy-pyrene-1,3,6-trisulfonic acid trisodium salt, enabling apoplastic pH dynamics to be assessed with high spatial resolution and accuracy.
- Ann-Kathrin Rößling
- Niklas Mayle
- Elke Barbez