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Showing 1–18 of 18 results
Advanced filters: Author: Ophir Shalem Clear advanced filters

  • CRISPR–Cas9 has been adopted as a powerful genome-editing technology in various species. By generating libraries of thousands of guide RNAs — which direct the Cas9 nuclease to chosen genomic loci — high-throughput genetic perturbations are now possible. This Review discusses the latest applications of CRISPR–Cas9 in mammalian functional genomics screens. It covers related genome-scale applications of Cas9 for either gene knockout or transcriptional modulation, and provides comparisons with complementary RNA interference (RNAi)-based approaches.

    • Ophir Shalem
    • Neville E. Sanjana
    • Feng Zhang
    Reviews
    Nature Reviews Genetics
    Volume: 16, P: 299-311

  • The authors find that TDP-43 loss of function—the pathology defining the neurodegenerative conditions ALS and FTD—induces novel mRNA polyadenylation events, which have different effects, including an increase in RNA stability, leading to higher protein levels.

    • Sam Bryce-Smith
    • Anna-Leigh Brown
    • Pietro Fratta
    ResearchOpen Access
    Nature Neuroscience
    Volume: 28, P: 2190-2200

  • Lundgren et al. show that in response to transient cold exposure, a distinct subpopulation of brown adipocytes carries out a lipogenic response involving production of acylcarnitines, which enables an improved thermogenic response to secondary cold exposure.

    • Patrick Lundgren
    • Prateek V. Sharma
    • Christoph A. Thaiss
    Research
    Nature Metabolism
    Volume: 5, P: 1691-1705

  • Functional genetic screening of mice and other mammals is exceedingly challenging. A CRISPR-based mutagenesis screen in mice has successfully revealed amino acids vital for protein function of the DND1 gene, missense mutations of which lead to defects in primordial germ cell development.

    • Yevgeniy V. Serebrenik
    • Ophir Shalem
    News & Views
    Nature Cell Biology
    Volume: 20, P: 1235-1237

  • How We Feel is a web and mobile-phone application for collecting de-identified self-reported COVID-19-related data. These data are used to map a diverse set of symptomatic, demographic, exposure and behavioural factors relevant to the ongoing pandemic.

    • William E. Allen
    • Han Altae-Tran
    • Xihong Lin
    Research
    Nature Human Behaviour
    Volume: 4, P: 972-982

  • The physical size of the commonly used Cas9 from Streptococcus pyogenes poses challenges for CRISPR-Cas genome editing systems that use the adeno-associated virus as a delivery vehicle; here, smaller Cas9 orthologues are characterized, and Cas9 from Staphylococcus aureus allowed targeting of the cholesterol regulatory gene Pcsk9 in the mouse liver.

    • F. Ann Ran
    • Le Cong
    • Feng Zhang
    Research
    Nature
    Volume: 520, P: 186-191

  • The authors describe a two-cell-type CRISPR screen to identify tumour-intrinsic genes that regulate the sensitivity of cancer cells to effector T cell function.

    • Shashank J. Patel
    • Neville E. Sanjana
    • Nicholas P. Restifo
    Research
    Nature
    Volume: 548, P: 537-542

  • We call upon the research community to standardize efforts to use daily self-reported data about COVID-19 symptoms in the response to the pandemic and to form a collaborative consortium to maximize global gain while protecting participant privacy.

    • Eran Segal
    • Feng Zhang
    • Paul Wilmes
    Comments & Opinion
    Nature Medicine
    Volume: 26, P: 1161-1165

  • A CRISPR-Cas9 approach is used to perform saturating mutagenesis of the human and mouse BCL11A enhancers, producing a map that reveals critical regions and specific vulnerabilities; BCL11A enhancer disruption is validated by CRISPR-Cas9 as a therapeutic strategy for inducing fetal haemoglobin by applying it in both mice and primary human erythroblast cells.

    • Matthew C. Canver
    • Elenoe C. Smith
    • Daniel E. Bauer
    Research
    Nature
    Volume: 527, P: 192-197

  • A secure framework that harmonizes storage and querying of clinical and genetic data using blockchain technology was developed to support combined genotype–phenotype queries, improving transparency into how and when health information is used.

    • Ahmed Elhussein
    • Ulugbek Baymuradov
    • Gamze Gürsoy
    ResearchOpen Access
    Nature Medicine
    Volume: 30, P: 3578-3589

  • ENL, identified in a genome-scale loss-of-function screen as a crucial requirement for proliferation of acute leukaemia, is required for leukaemic gene expression, and its YEATS chromatin-reader domain is essential for leukaemic growth.

    • Michael A. Erb
    • Thomas G. Scott
    • James E. Bradner
    Research
    Nature
    Volume: 543, P: 270-274

  • Analyses of the determinants of the specificity of Cas9 nuclease provide rules for selecting optimal target sites.

    • Patrick D Hsu
    • David A Scott
    • Feng Zhang
    Research
    Nature Biotechnology
    Volume: 31, P: 827-832