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Showing 1–7 of 7 results
Advanced filters: Author: Pablo Carravilla Clear advanced filters
  • Photoconversion of popular organic dyes results in blue-shifted emission and altered fluorescence lifetimes that can cause artifacts in quantitative microscopy. These can be avoided through proper labeling strategies such as using exchangeable dyes.

    • Anindita Dasgupta
    • Agnes Koerfer
    • Pablo Carravilla
    ResearchOpen Access
    Nature Methods
    Volume: 21, P: 1171-1174
  • Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment-sensitive probes.

    • Pablo Carravilla
    • Luca Andronico
    • Erdinc Sezgin
    Protocols
    Nature Protocols
    Volume: 20, P: 1976-2004
  • The Membrane-Proximal External Region (MPER) of the HIV Env gp41 subunit is a target for broadly neutralizing antibodies. Here, the authors apply super-resolution stimulated emission depletion (STED) microscopy on single virions and provide insights into how the MPER epitope is recognized.

    • Pablo Carravilla
    • Jakub Chojnacki
    • José L. Nieva
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-11
  • To become infectious, HIV-1 particles undergo a maturation process involving the clustering of envelope glycoprotein Env. Here, Chojnacki et al. employ super-resolution STED-FCS microscopy to study dynamics of Env molecules on HIV-1 particles and show that Env undergoes a maturation-induced increase in mobility.

    • Jakub Chojnacki
    • Dominic Waithe
    • Christian Eggeling
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-10
  • Deep learning enables restoration of noisy 2D and 3D STED images for long-term super-resolution imaging.

    • Vahid Ebrahimi
    • Till Stephan
    • Kyu Young Han
    ResearchOpen Access
    Communications Biology
    Volume: 6, P: 1-8