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Showing 1–3 of 3 results
Advanced filters: Author: Patrick Schindele Clear advanced filters

  • Manipulation of genetic exchange is an important objective of plant breeders. Using chromosome engineering to invert a 17.1 Mb fragment on chromosome 2 in Arabidopsis thaliana, meiotic recombination could be suppressed in nearly the entire chromosome.

    • Michelle Rönspies
    • Carla Schmidt
    • Holger Puchta
    Research
    Nature Plants
    Volume: 8, P: 1153-1159

  • This chromosome-engineering protocol generates heritable chromosomal rearrangements in A. thaliana; by combining SaCas9 with an egg-cell-specific promoter to facilitate heritable mutations, chromosomal rearrangements can be made and homozygous lines can be established.

    • Michelle Rönspies
    • Patrick Schindele
    • Holger Puchta
    Protocols
    Nature Protocols
    Volume: 17, P: 1332-1358