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Understanding functional miRNA–target interactions in vivo by site-specific genome engineering

Identifying miRNA response elements (MREs) within target mRNAs can be done computationally but the functional validation of putative MREs remains challenging. Here, Bassett et al. describe applications of genome engineering to target and assess the functional significance of MREs in different organisms and stages of development.

Andrew R. Bassett
Ghows Azzam
Tudor A. Fulga
ResearchOpen Access19 Aug 2014
Nature Communications

Volume: 5, P: 1-11
Genetic determinants of ulcerative colitis include the ECM1 locus and five loci implicated in Crohn's disease

Sheila A Fisher
Mark Tremelling
Jack Satsangi
Research27 Apr 2008
Nature Genetics

Volume: 40, P: 710-712
A systematic benchmark of Nanopore long-read RNA sequencing for transcript-level analysis in human cell lines

This analysis provides a collection of sequencing datasets generated from long-read and short-read RNA sequencing, serving as a valuable resource for transcriptome profiling.

Ying Chen
Nadia M. Davidson
Jonathan Göke
ResearchOpen Access13 Mar 2025
Nature Methods

Volume: 22, P: 801-812

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