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Showing 1–2 of 2 results
Advanced filters: Author: Philip P. Versluis Clear advanced filters

  • Tracking molecular modifications of engaged Pol II complexes is important for studying transcriptional regulation. Here, the authors combine run-on sequencing (PRO-seq) and immunoprecipitation, revealing dynamics of Pol II CTD phosphorylation at nucleotide-resolution.

    • Anniina Vihervaara
    • Philip Versluis
    • John T. Lis
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-19

  • Promoter-proximal pausing by RNA Pol II is a key aspect of how gene expression is transcriptionally regulated in higher eukaryotes. Here the authors show that only NELF-mediated pausing enforces a strict early checkpoint for Cdk9 by efficiently shutting down gene transcription following loss of Cdk9.

    • Michael DeBerardine
    • Gregory T. Booth
    • John T. Lis
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-15