CRISPR-Cas9 is an adaptive immune system that protects bacteria against invading DNA. Here, the authors show that the master virulence regulator CovR controls the CRISPR-Cas9 system in Streptococcus agalactiae, allowing transient increases in Cas9 off-target activity to overcome escape mutations in the target DNA, boost immune memory recall, and promote new memory acquisition.
- Adeline Pastuszka
- Maria-Vittoria Mazzuoli
- Arnaud Firon
