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Showing 1–6 of 6 results

Advanced filters: Author: Prayag Murawala Clear advanced filters

Publisher Correction: Application and optimization of CRISPR–Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum)

Ji-Feng Fei
Wilson Pak-Kin Lou
Elly M. Tanaka
Amendments and Corrections29 Jan 2019
Nature Protocols

Volume: 14, P: 2597
A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues

A ‘hybrid’ open-top light-sheet microscope is described, which can be used for broad multi-scale volumetric imaging of one or more large tissues, cleared with diverse protocols, and conveniently mounted on an array of sample holders.

Adam K. Glaser
Kevin W. Bishop
Jonathan T. C. Liu
Research11 May 2022
Nature Methods

Volume: 19, P: 613-619
A right-handed signalling pathway drives heart looping in vertebrates

Two parallel signalling pathways, driven by Nodal and BMP, respectively integrate left- and right-handed information that drives heart looping and morphogenesis, and are conserved between zebrafish, chicken and mouse.

Oscar H. Ocaña
Hakan Coskun
M. Angela Nieto
Research07 Sept 2017
Nature

Volume: 549, P: 86-90
Application and optimization of CRISPR–Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum)

This protocol describes strategies for targeted genome modification in axolotls. Eggs are injected with a CAS9–gRNA ribonucleoprotein complex, which allows for efficient generation of knockout and knock-in animals and immediate phenotypic analysis.

Ji-Feng Fei
Wilson Pak-Kin Lou
Elly M. Tanaka
Protocols14 Nov 2018
Nature Protocols

Volume: 13, P: 2908-2943
Tissue- and time-directed electroporation of CAS9 protein–gRNA complexes in vivo yields efficient multigene knockout for studying gene function in regeneration

Ji-Feng Fei
Dunja Knapp
Elly M Tanaka
ResearchOpen Access09 Jun 2016
npj Regenerative Medicine

Volume: 1, P: 1-9
Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination

Shahryar Khattak
Prayag Murawala
Elly M Tanaka
Protocols06 Feb 2014
Nature Protocols

Volume: 9, P: 529-540