Recently discovered type III-E CRISPR–Cas systems use the single Cas7-11 effector to cleave foreign RNA. Here the authors use cryo-electron microscopy and biochemical approaches to reveal the mechanisms underlying crRNA maturation, target RNA recognition and cleavage, and communication with the protease Csx29 by Cas7-11.
- Guimei Yu
- Xiaoshen Wang
- Heng Zhang
