The gut microbiome is key to health, yet its protein functions remain largely unexplored. Here, the authors present uMetaP, ultra-sensitive metaproteomics workflow that combines the timsTOF Ultra and FDR-validated de novo sequencing, boosting detection 5,000-fold and revealing gut inflammation targets.
- Feng Xian
- Malena Brenek
- David Gómez-Varela
