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Showing 1–3 of 3 results
Advanced filters: Author: Reynard Spiess Clear advanced filters

  • Engineering enzymes capable of performing chemical transformations requires high-throughput assays to screen activity. In this protocol, reactions in cell lysates are analyzed directly by desorption electrospray ionization (DESI) mass spectrometry.

    • Ruth Knox
    • Rachel Smith
    • Perdita E. Barran
    Protocols
    Nature Protocols
    Volume: 20, P: 3295-3313

  • Pablo Carbonell et al. present an automated pipeline for the discovery and optimization of biosynthetic pathways for microbial production of fine chemicals. They apply their pipeline to the production of the flavonoid (2S)-pinocembrin in Escherichia coli and show improvement of the pathway by 500-fold.

    • Pablo Carbonell
    • Adrian J. Jervis
    • Nigel S. Scrutton
    ResearchOpen Access
    Communications Biology
    Volume: 1, P: 1-10