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Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

Direct observation of G-quadruplexes (G4s) in live cells is challenging. Here the authors report a method to identify G4s within the nuclei of live and fixed cells using a fluorescent probe combined with fluorescence lifetime imaging microscopy.

Peter A. Summers
Benjamin W. Lewis
Ramon Vilar
ResearchOpen Access08 Jan 2021
Nature Communications

Volume: 12, P: 1-11

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Visualising G-quadruplex DNA dynamics in live cells by fluorescence lifetime imaging microscopy

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