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Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR

Quantifying genomic aberrations resulting from designer nucleases activity is essential for gene therapy clinical translation. Here, the authors present a modular digital PCR technique that profiles DNA repair precision and cut-repair cycles at the edited loci, exposing current evaluation biases.

Nathan White
John Alexander Chalk
Giandomenico Turchiano
ResearchOpen Access03 Nov 2025
Nature Communications

Volume: 16, P: 1-20
Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing

Prime editing is a next-generation approach for precision genome engineering. Here the authors design a nuclease-based prime editor that leverages DNA repair pathways for targeted genomic insertions.

Martin Peterka
Nina Akrap
Marcello Maresca
ResearchOpen Access24 Mar 2022
Nature Communications

Volume: 13, P: 1-9
A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo

SpCas9 unintended editing is a major concern. Here the authors report an off-target method using Duplex Sequencing with increased sensitivity for Cas9 mutation detection; they also identify a Cas9 variant of the II-B subfamily with intrinsic high fidelity (PsCas9) and see improved specificity.

Burcu Bestas
Sandra Wimberger
Marcello Maresca
ResearchOpen Access06 Sept 2023
Nature Communications

Volume: 14, P: 1-15
Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing

Low efficiency of target DNA integration remains a challenge in genome engineering. Here the authors perform large-scale compound library and genetic screens to identify targets that enhance gene editing: they see that combined DNA-PK and Polϴ inhibition with potent compounds increases editing efficiency and precision.

Sandra Wimberger
Nina Akrap
Marcello Maresca
ResearchOpen Access14 Aug 2023
Nature Communications

Volume: 14, P: 1-18
Dual inhibition of DNA-PK and Polϴ boosts precision of diverse prime editing systems

Prime editing can generate undesired editing outcomes. Here, authors describe a method for reducing both on-target and off-target imprecise editing outcomes of diverse prime editing modalities in different cell types with a combination of small molecules that inhibit both DNA-PK and PolΘ activity.

Louis C. Dacquay
Panagiotis Antoniou
Martin Peterka
ResearchOpen Access08 May 2025
Nature Communications

Volume: 16, P: 1-11
Universal toxin-based selection for precise genome engineering in human cells

Genome engineering in cell lines or human stem cells often has poor efficiency, limiting the development of research and therapeutic applications. Here, the authors use a toxin-based selection system for precise bi-allelic engineering in cells and in vivo.

Songyuan Li
Nina Akrap
Marcello Maresca
ResearchOpen Access21 Jan 2021
Nature Communications

Volume: 12, P: 1-14
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery

CRISPR/Cas9 technology has revolutionised the ability of scientists to make genetically modified cells and animal models. Here, the authors make a Tet-On genetically modified mouse using the Streptococcus pyogenes Cas9 and demonstrate that it can be used for generation of mice with lung cancer.

Anders Lundin
Michelle J. Porritt
Marcello Maresca
ResearchOpen Access29 Sept 2020
Nature Communications

Volume: 11, P: 1-16
Author Correction: Universal toxin-based selection for precise genome engineering in human cells

Songyuan Li
Nina Akrap
Marcello Maresca
Amendments and CorrectionsOpen Access10 May 2021
Nature Communications

Volume: 12, P: 1

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