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Showing 1–2 of 2 results
Advanced filters: Author: Sergey Naumenko Clear advanced filters

  • SpCas9 unintended editing is a major concern. Here the authors report an off-target method using Duplex Sequencing with increased sensitivity for Cas9 mutation detection; they also identify a Cas9 variant of the II-B subfamily with intrinsic high fidelity (PsCas9) and see improved specificity.

    • Burcu Bestas
    • Sandra Wimberger
    • Marcello Maresca
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-15

  • Ancient, asexual lineages are rare as a lack of recombination is usually an evolutionary dead end. Here, authors compare complete genomes of 11 individual bdelloid rotifers that suggest evidence of regular genetic exchange between individuals in a species that was previously thought to be asexual.

    • Olga A. Vakhrusheva
    • Elena A. Mnatsakanova
    • Alexey S. Kondrashov
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-17