Callaghan et al. present a combinatory approach to culturing harvested adult mouse cardiomyocytes (aCMs). Under traditional culture protocols, aCMs rapidly lose their phenotype and undergo cell death. With their protocol, the authors show aCMs remain viable and retain their phenotype for 7 days, enough time to do genetic manipulation and small molecule screening.
- Neal I. Callaghan
- Shin-Haw Lee
- Anthony O. Gramolini
