FRET has been used to study protein conformational changes but has never been applied to RNA aptamers. Here the authors develop a genetically encodable RNA aptamer-based FRET system on single-stranded RNA origami scaffolds, and demonstrate it can be used to study RNA conformational changes.
- Mette D. E. Jepsen
- Steffen M. Sparvath
- Ebbe S. Andersen
