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Showing 1–17 of 17 results
Advanced filters: Author: Stephan Preibisch Clear advanced filters

  • A connectome of the right optic lobe from a male fruitfly is presented together with an extensive collection of genetic drivers matched to a comprehensive neuron-type catalogue.

    • Aljoscha Nern
    • Frank Loesche
    • Michael B. Reiser
    ResearchOpen Access
    Nature
    Volume: 641, P: 1225-1237

  • Bharathan et al. discover that the endoplasmic reticulum associates with keratin intermediate filaments and desmosomal cell–cell junctions, and that desmosomes and the keratin cytoskeleton regulate the distribution, dynamics and function of the endoplasmic reticulum network.

    • Navaneetha Krishnan Bharathan
    • William Giang
    • Andrew P. Kowalczyk
    Research
    Nature Cell Biology
    Volume: 25, P: 823-835

  • mRNA translation is tightly regulated to preserve cellular homeostasis. Here live-cell spectroscopy and single-particle tracking are combined to interrogate the binding dynamics of endogenous initiation factors to mRNAs 5’cap, revealing the sequence of translation initiation factors assembly and disassembly; and the clustering of translation in neurons.

    • Valentina Gandin
    • Brian P. English
    • Robert H. Singer
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-15

  • RS-FISH is a user-friendly software for accurate spot detection that is applicable to smFISH experiments, spatial transcriptomics, and spatial genomics. The approach enables fast spot detection in even very large volumetric datasets.

    • Ella Bahry
    • Laura Breimann
    • Stephan Preibisch
    ResearchOpen Access
    Nature Methods
    Volume: 19, P: 1563-1567

  • A graphical processing unit implementation of an efficient Bayesian-based multiview deconvolution method brings the resolution and contrast advantages of multiview deconvolution to more users of light-sheet fluorescence microscopy.

    • Stephan Preibisch
    • Fernando Amat
    • Pavel Tomancak
    Research
    Nature Methods
    Volume: 11, P: 645-648

  • Presented is an overview of the image-analysis software platform Fiji, a distribution of ImageJ that updates the underlying ImageJ architecture and adds modern software design elements to expand the capabilities of the platform and facilitate collaboration between biologists and computer scientists.

    • Johannes Schindelin
    • Ignacio Arganda-Carreras
    • Albert Cardona
    Reviews
    Nature Methods
    Volume: 9, P: 676-682

  • For two hundred years, scientists have noticed that the appearance of embryos in related species converge in their appearance mid-way in development, diverging thereafter. But is this 'phylotypic stage' real, and how is it connected with the genetic basis of development? Here, a method linking the genes transcribed at various stages of development (the transcriptome) with the evolutionary history of those genes is used. Genes transcribed in the phylotypic stage are, in evolutionary terms, the oldest and most conserved. This suggests that the phylotypic stage does represent the body plans of related species at their most unadorned, selection having sculpted the earlier and later stages of embryonic form to suit the particulars of each creature.

    • Alex T. Kalinka
    • Karolina M. Varga
    • Pavel Tomancak
    Research
    Nature
    Volume: 468, P: 811-814

  • This Perspective focuses on experimental techniques developed for circRNA studies, and provides practical guidelines for performing circRNA research.

    • Anne F. Nielsen
    • Albrecht Bindereif
    • Jørgen Kjems
    Reviews
    Nature Methods
    Volume: 19, P: 1208-1220

  • Light sheet fluorescence microscopy (LSFM) is a technique that uses a thin sheet of light for illumination, allowing optical sectioning of the sample. In this Primer, Stelzer et al. outline the fundamental concepts behind LSFM, discuss the different experimental set-ups for light sheet microscopes and detail steps for processing LSFM images. The Primer also describes the range of applications for this technique across the biological sciences and concludes by discussing advances for enhancing imaging depth and resolution.

    • Ernst H. K. Stelzer
    • Frederic Strobl
    • Reto Fiolka
    Reviews
    Nature Reviews Methods Primers
    Volume: 1, P: 1-25