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S9 protease WprP₂ catalyzes uniform cleavage on the precursor peptide in RiPP biosynthesis

Ribosomally synthesized and post-translationally modified peptides (RiPPs) require precise proteolytic cleavage to generate bioactive natural products, yet the diversity of serine proteases involved remains underexplored. Here, the authors identify and characterize the serine protease WprP₂ from Streptomyces venezuelae NPDC049867, revealing its unique cleavage activity on precursor peptides WprA₂ involved in the biosynthesis of cyclophane RiPP natural products.

Jabal Rahmat Haedar
Abujunaid Habib Khan
Chin-Soon Phan
ResearchOpen Access29 Jan 2026
Communications Chemistry

P: 1-6
Author Correction: Fused radical SAM and αKG-HExxH domain proteins contain a distinct structural fold and catalyse cyclophane formation and β-hydroxylation

Yohei Morishita
Suze Ma
Brandon I. Morinaka
Amendments and Corrections17 Mar 2025
Nature Chemistry

Volume: 17, P: 624
Fused radical SAM and αKG-HExxH domain proteins contain a distinct structural fold and catalyse cyclophane formation and β-hydroxylation

Radical SAM (rSAM) and 2-His-1-carboxylate enzymes are known to co-occur in RiPP biosynthetic pathways. Here we show the fusion of these enzymes in a single protein where the rSAM enzyme catalyzes cyclophane formation. The 2-His-1-carboxylate enzymes—termed αKG-HExxH—are α-ketoglutarate non-haem iron enzymes that harbour a distinct fold and catalyse β-hydroxylation.

Yohei Morishita
Suze Ma
Brandon I. Morinaka
Research18 Sept 2024
Nature Chemistry

Volume: 16, P: 1882-1893
Radical SAM-dependent ether crosslink in daropeptide biosynthesis

Darobactin is a ribosomally synthesized and post-translationally modified peptide featuring a unique scaffold and potent activity against Gram-negative bacteria. Here, the authors identify darobactin synthase DarE as responsible for the bicyclic scaffold formation and propose the name daropeptide for this growing class of enzymes.

Sijia Guo
Shu Wang
Qi Zhang
ResearchOpen Access29 Apr 2022
Nature Communications

Volume: 13, P: 1-9
Genome-wide linkage analysis combined with genome sequencing in large families with intracranial aneurysms

Mark K. Bakker
Suze Cobyte
Ynte M. Ruigrok
ResearchOpen Access01 Mar 2022
European Journal of Human Genetics

Volume: 30, P: 833-840
The definition of low wall shear stress and its effect on plaque progression estimation in human coronary arteries

Eline M. J. Hartman
Giuseppe De Nisco
Jolanda J. Wentzel
ResearchOpen Access11 Nov 2021
Scientific Reports

Volume: 11, P: 1-11
Tumour immune characterisation of primary triple-negative breast cancer using automated image quantification of immunohistochemistry-stained immune cells

Suze Roostee
Daniel Ehinger
Mattias Aine
ResearchOpen Access13 Sept 2024
Scientific Reports

Volume: 14, P: 1-19

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S9 protease WprP2 catalyzes uniform cleavage on the precursor peptide in RiPP biosynthesis

Author Correction: Fused radical SAM and αKG-HExxH domain proteins contain a distinct structural fold and catalyse cyclophane formation and β-hydroxylation

Fused radical SAM and αKG-HExxH domain proteins contain a distinct structural fold and catalyse cyclophane formation and β-hydroxylation

Radical SAM-dependent ether crosslink in daropeptide biosynthesis

Genome-wide linkage analysis combined with genome sequencing in large families with intracranial aneurysms

The definition of low wall shear stress and its effect on plaque progression estimation in human coronary arteries

Tumour immune characterisation of primary triple-negative breast cancer using automated image quantification of immunohistochemistry-stained immune cells

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S9 protease WprP₂ catalyzes uniform cleavage on the precursor peptide in RiPP biosynthesis