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Showing 1–5 of 5 results

Advanced filters: Author: Thomas Cherrier Clear advanced filters

Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins

It can be challenging to obtain meaningful and accurate structural information for air-sensitive proteins. This protocol describes the application of customized vacuum manifold and anaerobic chamber setups for the purification and cryo-electron microscopy analysis of air-sensitive nitrogenase enzymes.

Rebeccah A. Warmack
Belinda B. Wenke
Douglas C. Rees
Protocols04 Apr 2024
Nature Protocols

Volume: 19, P: 2026-2051
Dephosphorylation of HDAC4 by PP2A-Bδ unravels a new role for the HDAC4/MEF2 axis in myoblast fusion

Alexandra Veloso
Maud Martin
Franck Dequiedt
ResearchOpen Access04 Jul 2019
Cell Death & Disease

Volume: 10, P: 1-16
Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy

A protocol for differentiating mouse and human pluripotent stem cells into muscle.

Jérome Chal
Masayuki Oginuma
Olivier Pourquié
Research01 Sept 2015
Nature Biotechnology

Volume: 33, P: 962-969
HUMANIN produced by human efferocytic macrophages promotes the resolution of inflammation

Mélissa Maraux
Mathieu Vetter
Thomas Cherrier
ResearchOpen Access28 Aug 2025
Cell Death & Disease

Volume: 16, P: 1-13
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro

This protocol uses dual modulation of Wnt and BMP signaling pathways to differentiate human pluripotent stem cells into striated, millimeter-long muscle fibers and satellite-like cells in vitro without the need to introduce genetic material or cell sorting.

Jérome Chal
Ziad Al Tanoury
Olivier Pourquié
Protocols01 Sept 2016
Nature Protocols

Volume: 11, P: 1833-1850