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Showing 1–13 of 13 results
Advanced filters: Author: Thomas Schlichthaerle Clear advanced filters

  • Single-molecule localisation microscopy is limited by low labeling and detection efficiencies of the molecular probes. Here the authors report a framework to obtain absolute molecular quantities on a true molecular scale; the data reveal a ternary adhesion complex underlying cell-matrix adhesion.

    • Lisa S. Fischer
    • Christoph Klingner
    • Carsten Grashoff
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-10

  • Relatively little is known about cell-matrix interactions and the intracellular transduction of an initial ligand-receptor binding event on the single-molecule level. Here authors combine ligand-decorated DNA tension sensors with DNA-PAINT super-resolution microscopy to study the mechanical engagement of single integrin receptors and the downstream influence on actin bundling.

    • Thomas Schlichthaerle
    • Caroline Lindner
    • Ralf Jungmann
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-8

  • Immune receptors regulate immune responses and are key cancer immunotherapy targets. Here, the authors designed helical concave scaffolds to bind convex sites in immune receptors, creating high-affinity protein binders for TGFβRII, CTLA-4, and PD-L1. Co-crystal structures confirmed their therapeutic potential.

    • Wei Yang
    • Derrick R. Hicks
    • David Baker
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-12

  • Computationally designed genetically encoded proteins can be used to target surface proteins, thereby triggering endocytosis and subsequent intracellular degradation, activating signalling or increasing cellular uptake in specific tissues.

    • Buwei Huang
    • Mohamad Abedi
    • David Baker
    ResearchOpen Access
    Nature
    Volume: 638, P: 796-804

  • A synthetic receptor platform that enables mammalian cells to respond to soluble factors allows specific signalling networks to be precisely controlled, with a variety of therapeutic applications.

    • Dan I. Piraner
    • Mohamad H. Abedi
    • Kole T. Roybal
    ResearchOpen Access
    Nature
    Volume: 638, P: 805-813

  • The interphase centrosome protein AKNA is necessary and sufficient for the organization of centrosomal microtubules, mediates delamination in the formation of the subventricular zone and regulates exit from this zone.

    • Germán Camargo Ortega
    • Sven Falk
    • Magdalena Götz
    Research
    Nature
    Volume: 567, P: 113-117

  • Single-molecule localization microscopy relies on stochastic blinking events, treated as independent events without assignment to a particular emitter. Here, BaGoL takes low precision localizations generated from multiple emitter blinkings during DNAPAINT and dSTORM and finds the underlying emitter positions with high precision.

    • Mohamadreza Fazel
    • Michael J. Wester
    • Keith A. Lidke
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-11

  • Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.

    • Hansjörg Götzke
    • Markus Kilisch
    • Steffen Frey
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-12

  • Basnet et al. discover that in vitro SSNA1 forms fibrils that attach along protofilaments and guide these to grow away from microtubules, forming templates for branched microtubules. SSNA1 mutations that perturb this process lead to defective axon branching in primary neurons.

    • Nirakar Basnet
    • Hana Nedozralova
    • Naoko Mizuno
    Research
    Nature Cell Biology
    Volume: 20, P: 1172-1180

  • The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off-the-shelf fluorescence microscopy hardware and reagents.

    • Susanne C. M. Reinhardt
    • Luciano A. Masullo
    • Ralf Jungmann
    ResearchOpen Access
    Nature
    Volume: 617, P: 711-716

  • Point spread function (PSF) splitting with the ‘Circulator’, which encodes the fluorophore emission band into the PSF, improves the information content of fluorescence microscopy and enables improved super-resolution imaging and single-particle tracking.

    • Robin Van den Eynde
    • Fabian Hertel
    • Peter Dedecker
    Research
    Nature Methods
    Volume: 21, P: 1801-1805

  • In DNA-PAINT, transient binding of dye-labeled oligonucleotides to their target strands creates the ‘blinking’ required for stochastic nanoscopy. This protocol describes how to apply DNA-PAINT, from sample preparation to data processing.

    • Joerg Schnitzbauer
    • Maximilian T Strauss
    • Ralf Jungmann
    Protocols
    Nature Protocols
    Volume: 12, P: 1198-1228