Characterizing protein-ligand interactions is crucial for drug development, however, studying weak binders like fragments remains challenging. Here, the authors demonstrate that Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS) can effectively characterize fragment binding to Cyclophilin D, offering a valuable tool for fragment-based drug discovery, complementary to other higher resolution techniques.
- Catarina F. Malta
- Diana O. Silva
- Alessio Bortoluzzi
