Femtosecond X-ray free electron laser (XFEL) pulses enable structure analysis of single particles by diffraction, outrunning radiation damage and avoiding ensemble averaging. Here, the authors inject single lipid vesicles sequentially into an XFEL beam, and analyze the small-angle scattering for each hit to retrieve the radial electron density profile at a resolution high enough to resolve the lipid bilayer.
- Charlotte Neuhaus
- Moritz L. Stammer
- Tim Salditt
