In living systems, ATP provides an energetic driving force for protein synthesis and modification. Now, an engineered enzymatic tool has been developed for high-yield, ATP-driven protein bioconjugation through versatile thioester intermediates. This method mimics biological strategies for peptide bond formation to enable precision chemical modification of proteins in vitro.
- Clara L. Frazier
- Debashrito Deb
- Amy M. Weeks
