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A plasmid-based multigene expression system for mammalian cells

Fluorescent proteins are widely used in molecular biology to visualize protein expression and localization. Here, Krizet al. describe an efficient and flexible modular plasmid-based eukaryotic expression cloning strategy for the homogeneous expression of several fluorescent proteins in one cell.

Andrijana Kriz
Katharina Schmid
Philipp Berger
Research16 Nov 2010
Nature Communications

Volume: 1, P: 1-6
Real-time visualization of STAT activation in live cells using genetically encoded biosensors

The signal transducer and activator of transcription (STAT) family proteins are attractive drug targets but tools to monitor their activation are lacking. Now, STAT biosensors have been developed for real-time tracking in live cells and are applied to screen inhibitors and investigate the effects of cancer-associated mutations.

Thi A. N. Nguyen
Roman Meledin
Onur Boyman
ResearchOpen Access01 Sept 2025
Nature Chemical Biology

P: 1-10
Multigenerational cell tracking of DNA replication and heritable DNA damage

A multigenerational single-cell tracking approach provides a framework to dissect phenotypic plasticity at the single-cell level, offering insights into cellular processes that may resemble early events during cancer development.

Andreas Panagopoulos
Merula Stout
Matthias Altmeyer
ResearchOpen Access21 May 2025
Nature

Volume: 642, P: 785-795
The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

The mesoSPIM is an open hardware axially scanned light-sheet microscope for the rapid imaging of large cleared samples with isotropic resolution.

Fabian F. Voigt
Daniel Kirschenbaum
Fritjof Helmchen
Research16 Sept 2019
Nature Methods

Volume: 16, P: 1105-1108
Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

The demand to image large biological samples at high resolution requires improvement in current light-sheet microscopy tools. Here, the authors present an improved, benchtop mesoSPIM with a significantly increased field-of-view, improved resolution and improved throughput.

Nikita Vladimirov
Fabian F. Voigt
Fritjof Helmchen
ResearchOpen Access27 Mar 2024
Nature Communications

Volume: 15, P: 1-16
Atomically flat single-crystalline gold nanostructures for plasmonic nanocircuitry

Polycrystalline substrates are a hindrance to the realization of high-definition plasmonic nanostructures. In this paper the authors chemically grow large and thin gold single crystals, and show that they can be coupled with top-down fabrication methods to produce high-quality nanostructures with good optical properties.

Jer-Shing Huang
Victor Callegari
Bert Hecht
Research21 Dec 2010
Nature Communications

Volume: 1, P: 1-8
Direct imaging of uncoated biological samples enables correlation of super-resolution and electron microscopy data

José María Mateos
Gery Barmettler
Urs Ziegler
ResearchOpen Access02 Aug 2018
Scientific Reports

Volume: 8, P: 1-8
Topographic contrast of ultrathin cryo-sections for correlative super-resolution light and electron microscopy

José María Mateos
Bruno Guhl
Urs Ziegler
ResearchOpen Access26 Sept 2016
Scientific Reports

Volume: 6, P: 1-8
Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1

Lloyd C. Trotman
Nicole Mosberger
Urs F. Greber
Research02 Nov 2001
Nature Cell Biology

Volume: 3, P: 1092-1100
Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections

Luis E. Villegas-Hernández
Vishesh Dubey
Balpreet Singh Ahluwalia
ResearchOpen Access24 Feb 2022
Light: Science & Applications

Volume: 11, P: 1-17
Fenretinide induces a new form of dynamin-dependent cell death in pediatric sarcoma

Eva Brack
Marco Wachtel
Beat W. Schäfer
Research06 Mar 2020
Cell Death & Differentiation

Volume: 27, P: 2500-2516
The iron chelator Deferasirox causes severe mitochondrial swelling without depolarization due to a specific effect on inner membrane permeability

Esther M. Gottwald
Claus D. Schuh
Andrew M. Hall
ResearchOpen Access31 Jan 2020
Scientific Reports

Volume: 10, P: 1-15
Environmental marine pathogen isolation using mesocosm culture of sharpsnout seabream: striking genomic and morphological features of novel Endozoicomonas sp.

Pantelis Katharios
Helena M. B. Seth-Smith
Lloyd Vaughan
ResearchOpen Access07 Dec 2015
Scientific Reports

Volume: 5, P: 1-13
Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh

Aurélien Rizk
Grégory Paul
Ivo F Sbalzarini
Protocols13 Feb 2014
Nature Protocols

Volume: 9, P: 586-596
Genome-wide association study reveals new insights into the heritability and genetic correlates of developmental dyslexia

Alessandro Gialluisi
Till F. M. Andlauer
Gerd Schulte-Körne
ResearchOpen Access14 Oct 2020
Molecular Psychiatry

Volume: 26, P: 3004-3017
Genome-wide association scan identifies new variants associated with a cognitive predictor of dyslexia

Alessandro Gialluisi
Till F. M. Andlauer
Gerd Schulte-Körne
ResearchOpen Access11 Feb 2019
Translational Psychiatry

Volume: 9, P: 1-15
Rapid adaptation drives invasion of airway donor microbiota by Pseudomonas after lung transplantation

M. Beaume
T. Köhler
Patrick Yerly
ResearchOpen Access17 Jan 2017
Scientific Reports

Volume: 7, P: 1-10
CRTC2 polymorphism as a risk factor for the incidence of metabolic syndrome in patients with solid organ transplantation

L Quteineh
P-Y Bochud
Patrick Yerly
Research08 Dec 2015
The Pharmacogenomics Journal

Volume: 17, P: 69-75
Emerging pathogens of gilthead seabream: characterisation and genomic analysis of novel intracellular β-proteobacteria

Helena M B Seth-Smith
Nancy Dourala
Lloyd Vaughan
Research05 Feb 2016
The ISME Journal

Volume: 10, P: 1791-1803

Search

A plasmid-based multigene expression system for mammalian cells

Real-time visualization of STAT activation in live cells using genetically encoded biosensors

Multigenerational cell tracking of DNA replication and heritable DNA damage

The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples

Atomically flat single-crystalline gold nanostructures for plasmonic nanocircuitry

Direct imaging of uncoated biological samples enables correlation of super-resolution and electron microscopy data

Topographic contrast of ultrathin cryo-sections for correlative super-resolution light and electron microscopy

Import of adenovirus DNA involves the nuclear pore complex receptor CAN/Nup214 and histone H1

Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections

Fenretinide induces a new form of dynamin-dependent cell death in pediatric sarcoma

The iron chelator Deferasirox causes severe mitochondrial swelling without depolarization due to a specific effect on inner membrane permeability

Environmental marine pathogen isolation using mesocosm culture of sharpsnout seabream: striking genomic and morphological features of novel Endozoicomonas sp.

Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh

Genome-wide association study reveals new insights into the heritability and genetic correlates of developmental dyslexia

Genome-wide association scan identifies new variants associated with a cognitive predictor of dyslexia

Rapid adaptation drives invasion of airway donor microbiota by Pseudomonas after lung transplantation

CRTC2 polymorphism as a risk factor for the incidence of metabolic syndrome in patients with solid organ transplantation

Emerging pathogens of gilthead seabream: characterisation and genomic analysis of novel intracellular β-proteobacteria

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