Proximity labeling allows proteomic mapping of subcellular structures. Here, Sroka et al. develop in situ APEX activation (iAPEX) to overcome limitations of labeling specificity and H2O2 toxicity, applying their approach to reveal cilia proteomes and expand proteomic access across organelles, cell types and organisms.
- Tommy J. Sroka
- Lea K. Sanwald
- David U. Mick
