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Showing 1–13 of 13 results
Advanced filters: Author: Vassilis Roukos Clear advanced filters

  • Roukos and Longo recount the findings of two 1982 papers, which proved that chromosomes and spaghetti are not alike.

    • Gabriel M. C. Longo
    • Vassilis Roukos
    Research Highlights
    Nature Reviews Molecular Cell Biology
    Volume: 22, P: 508

  • BreakTag is a scalable next-generation sequencing-based method for the unbiased characterization of programmable nucleases and guide RNAs that allows off-target and nuclease activity assessment, as well as the characterization of scission profiles.

    • Gabriel M. C. Longo
    • Sergi Sayols
    • Vassilis Roukos
    Protocols
    Nature Protocols
    P: 1-40

  • The assembly of polymerized actin with motor proteins at DNA breaks in the nucleus supports the mobility and repair of DNA. This finding reveals a layer of regulation that helps to preserve genome integrity.

    • Vassilis Roukos
    News & Views
    Nature
    Volume: 559, P: 35-37

  • Repair of a chromosome break can result in part of a chromosome attaching to a different chromosome, causing gene deregulation and disease. Roukos and Misteli discuss the spatial aspect of chromosome translocation and the role of DNA repair pathways in this process.

    • Vassilis Roukos
    • Tom Misteli
    Reviews
    Nature Cell Biology
    Volume: 16, P: 293-300

  • It remains challenging to characterise somatic copy number alterations (SCNAs) in tumors and the surrounding tissues with spatial and single-cell resolution. Here, the authors develop the scCUTseq approach to characterise SCNAs from single cells in multi-region prostate cancer samples and identify pseudo-diploid cells and subclones.

    • Ning Zhang
    • Luuk Harbers
    • Nicola Crosetto
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-17

  • During the repair of DNA breaks by homologous recombination, damaged DNA seeks out intact, similar in sequence segments to use as a template. In this issue, Piazza, Bordelet and colleagues describe three-dimensional chromosome architecture remodelling during homologous recombination and show that cohesin restricts homology search in cis, independently of sister chromatid cohesion.

    • Argyris Papantonis
    • Vassilis Roukos
    News & Views
    Nature Cell Biology
    Volume: 23, P: 1112-1114

  • HIV-1 usually targets active genes and integrates near the nuclear pore compartment. Here the authors show that recurrently targeted genes are proximal to super-enhancer genomic elements, which cluster in specific spatial compartments of the T cell nucleus, suggesting a role for nuclear organisation in viral infection.

    • Bojana Lucic
    • Heng-Chang Chen
    • Marina Lusic
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-12

  • Transcription can pose a threat to genomic instability through the formation of R-loops, which are RNA–DNA hybrids with a displaced non-template DNA strand. Here the authors mapped the R-loop proximal proteome in human cells and identified a role of the tumor suppressor DDX41 in opposing R-loop and double strand DNA break accumulation in gene promoters.

    • Thorsten Mosler
    • Francesca Conte
    • Petra Beli
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-17

  • This protocol describes experimental and computational procedures for genome-wide detection of endogenous and induced DNA double-strand breaks (DSBs) in any cell type or tissue that can be brought into suspension.

    • Britta A. M. Bouwman
    • Federico Agostini
    • Nicola Crosetto
    Protocols
    Nature Protocols
    Volume: 15, P: 3894-3941

  • This Protocol from the Misteli lab describes how to determine the cell cycle stage of each individual cell in a population by using fluorescence microscopy and automated image analysis software.

    • Vassilis Roukos
    • Gianluca Pegoraro
    • Tom Misteli
    Protocols
    Nature Protocols
    Volume: 10, P: 334-348