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Showing 1–6 of 6 results

Advanced filters: Author: Vikram Pattanayak Clear advanced filters

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

A high-fidelity variant of Streptococcus pyogenes CRISPR–Cas9 is reported that lacks detectable off-target events as assessed by genome-wide break capture and targeted sequencing methods.

Benjamin P. Kleinstiver
Vikram Pattanayak
J. Keith Joung
Research06 Jan 2016
Nature

Volume: 529, P: 490-495
Small molecule–triggered Cas9 protein with improved genome-editing specificity

Post-translational regulation of Cas9 activity may improve the specificity of genomic targeting. A modified version of Cas9 with an insertion of a small molecule–regulated intein allows temporal control of Cas9 activity and reduces off-target activity.

Kevin M Davis
Vikram Pattanayak
David R Liu
Research06 Apr 2015
Nature Chemical Biology

Volume: 11, P: 316-318
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

In vitro selection and high-throughput sequencing measure the sequence specificity of cleavage by gRNA-Cas9 complexes.

Vikram Pattanayak
Steven Lin
David R Liu
Research11 Aug 2013
Nature Biotechnology

Volume: 31, P: 839-843
Revealing off-target cleavage specificities of zinc-finger nucleases by in vitro selection

An in vitro method for examining cleavage patterns of zinc-finger nucleases (ZFNs) identifies previously unknown off-target cleavage sites. Some of the sites are present in the human genome and show evidence for ZFN-induced cleavage in cultured human cell lines.

Vikram Pattanayak
Cherie L Ramirez
David R Liu
Research07 Aug 2011
Nature Methods

Volume: 8, P: 765-770
Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity

High-throughput in vitro analysis of TALE nuclease (TALEN) cleavage specificity gives insight into the mechanism of TALE binding and allows the design of TALENs with lower off-target activity.

John P Guilinger
Vikram Pattanayak
David R Liu
Research16 Feb 2014
Nature Methods

Volume: 11, P: 429-435
Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”

Caleb A Lareau
Kendell Clement
Luca Pinello
Correspondence30 Mar 2018
Nature Methods

Volume: 15, P: 238-239