Ohannesian, Mallick et al. demonstrate label-free counting and detection of microRNA cargoes for single small extracellular vesicles (sEVs), revealing elevated sEV counts and distinct molecular contents in samples from patients with cancer. Using a simple threshold of sEV count in a blind test, classification of cancer vs. healthy samples is achieved with high sensitivity and specificity.
- Nareg Ohannesian
- Mohammad Sadman Mallick
- Wei-Chuan Shih
