The toolbox of artificial fluorescent proteins can be expanded by engineering mimics of the molecular rotor-based fluorophore found in the green fluorescent protein (GFP) into diverse protein scaffolds. Now, by genetically encoding mimics of the GFP fluorophore, any protein of interest can be modified to fluoresce either under select circumstances or always (when folded).
- Liming Hu
- Wenbing Cao
- Tao Liu
