New enzyme catalyst consisting of pyrenecarboxaldehyde (PCA) and glucose oxidase (GOx) immobilized on polyethyleneimine and carbon nanotube (CNT/PEI/[PCA/GOx]) is developed with performance and stability evaluations of enzymatic biofuel cell (EBC) using the new catalyst. By employment of PCA, the amount of immobilized GOx increases and electron transfer of CNT/PEI/[PCA/GOx] is facilitated. With that, superior EBC performance, long-lasting stability and excellent catalytic activities are gained. Such results are attributed to effects of (i) electron collection by hydrophobic interactions, (ii) electron transfer by π-conjugated bonds and (iii) enzyme stabilization by π-hydrogen bonds. Validity of such three positive effects is proved by various measurements.
- Marcelinus Christwardana
- Yongjin Chung
- Yongchai Kwon
