Nature Search

Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA

CRISPR systems are powerful tools for gene editing and diagnostics, but their regulation is challenging. Here, the authors present DNACas, a light-controlled method using photocleavable phosphorothioate DNA to modulate CRISPR activity, enabling precise gene editing and one-pot diagnostic detection.

Menglu Hu
Bingni Zhang
Xiaoming Zhou
ResearchOpen Access01 Jul 2025
Nature Communications

Volume: 16, P: 1-17
A substrate-multiplexed platform for profiling enzymatic potential of plant family 1 glycosyltransferases

Most enzymes involved in plant natural products biosynthesis remain uncharacterized. Here, the authors establish a screening pipeline and apply it to family 1 glycosyltransferases to reveal their promiscuity and preference for planar, hydroxylated aromatic substrates.

Sasilada Sirirungruang
Vincent Blay
Patrick M. Shih
ResearchOpen Access10 Jul 2025
Nature Communications

Volume: 16, P: 1-12
Self-optimizing, highly surface-active layered metal dichalcogenide catalysts for hydrogen evolution

Metal dichalcogenides are promising electrocatalysts for hydrogen evolution, but more active and stable materials are desired. Here the authors demonstrate that H-TaS₂ and H-NbS₂ possess high basal-plane activity that increases with cycling through changes in the morphology of the catalysts.

Yuanyue Liu
Jingjie Wu
Boris I. Yakobson
Research31 Jul 2017
Nature Energy

Volume: 2, P: 1-7
Compact zinc finger architecture utilizing toxin-derived cytidine deaminases for highly efficient base editing in human cells

The most recent class of base editors utilize DddAtox, a deaminase domain that can act upon double-stranded DNA. Here the authors target DddAtox fragments and a FokI-based nickase to the human CIITA gene by fusing these domains to arrays of engineered zinc fingers; they also identify a variety of DddAtox orthologues.

Friedrich Fauser
Bhakti N. Kadam
Jeffrey C. Miller
ResearchOpen Access15 Feb 2024
Nature Communications

Volume: 15, P: 1-11
Functional footprinting of regulatory DNA

Tiling of regulatory DNA with mutations introduced by genome editing nucleases and linking the resulting alleles to a phenotypic readout allows the precise determination of functional sequence motifs within these regions.

Jeff Vierstra
Andreas Reik
John A Stamatoyannopoulos
Research31 Aug 2015
Nature Methods

Volume: 12, P: 927-930
General synthesis of high-entropy single-atom nanocages for electrosynthesis of ammonia from nitrate

Converting nitrate from waste sources into ammonia provides an effective method for both wastewater treatment and ammonia production. Here the authors report a scalable solution-phase synthesis of high-entropy single-atom nanocage catalysts for efficient nitrate-to-ammonia conversion.

Sishuang Tang
Minghao Xie
Guihua Yu
ResearchOpen Access13 Aug 2024
Nature Communications

Volume: 15, P: 1-12
Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

The detection of pathogens in food and clinical samples remains a challenge. Here, Shen et al. present a detection system, involving a combination of nucleic acid-based allosteric probes and CRISPR-Cas13a components, that can detect very low numbers of a bacterial pathogen in milk and serum samples without isolation.

Jinjin Shen
Xiaoming Zhou
Da Xing
ResearchOpen Access14 Jan 2020
Nature Communications

Volume: 11, P: 1-10
Enhancing gene editing specificity by attenuating DNA cleavage kinetics

Engineered zinc finger nucleases achieve 98% knockout of a target gene in human T cells without detectable off-target activity.

Jeffrey C. Miller
Deepak P. Patil
Edward J. Rebar
Research29 Jul 2019
Nature Biotechnology

Volume: 37, P: 945-952

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