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Showing 1–5 of 5 results

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Correction: Corrigendum: The PURE system for the cell-free synthesis of membrane proteins

Yutetsu Kuruma
Takuya Ueda
Amendments and Corrections25 Feb 2016
Nature Protocols

Volume: 11, P: 616
Artificial photosynthetic cell producing energy for protein synthesis

Artificial cells need to be supplied with ATP as they lack internal systems of energy generation. Here the authors reconstruct ATP synthase and bacteriorhodopsins for light-driven ATP generation, powering transcription and translation.

Samuel Berhanu
Takuya Ueda
Yutetsu Kuruma
ResearchOpen Access22 Mar 2019
Nature Communications

Volume: 10, P: 1-10
CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo

Katsuhiro Sawasato
Ryo Sato
Ken-ichi Nishiyama
ResearchOpen Access04 Feb 2019
Scientific Reports

Volume: 9, P: 1-15
The PURE system for the cell-free synthesis of membrane proteins

This protocol enables cell-free synthesis of membrane proteins using the PURE system, subsequent quantification of products and analyses of membrane localization efficiency, product orientation, and complex formation in the membrane.

Yutetsu Kuruma
Takuya Ueda
Protocols13 Aug 2015
Nature Protocols

Volume: 10, P: 1328-1344
Phospholipid synthesis inside phospholipid membrane vesicles

A giant vesicle system combines fatty acid synthesis pathways, CoA recycling, and cell-free acyltransferase gene expression to perform phospholipid synthesis inside.

Sumie Eto
Rumie Matsumura
Yutetsu Kuruma
ResearchOpen Access27 Sept 2022
Communications Biology

Volume: 5, P: 1-11