Pei and colleagues show that introduction of the pluripotency reprogramming factors in sequence (Oct4–Klf4, c-Myc and finally Sox-2), rather than introducing them all together, increases reprogramming efficiency. This sequential delivery activates the epithelial-to-mesenchymal transition (EMT) before the mesenchymal-to-epithelial transition (MET), which was previously reported to occur during reprogramming. The authors also show that addition of EMT modulators influences reprogramming in a similar manner.
- Xiaopeng Liu
- Hao Sun
- Hui Zheng
