Combining enzymes from different pathways in nature could enable de novo peptide design, but determining enzyme-specificity rules is non-trivial. Here a biophysical model combining enzymes sourced from bacterial ribosomally synthesized and post-translationally modified peptide (RiPP) gene clusters was generated to formalize enzyme-specificity rules and create peptide scaffolds with defined post-translational modifications.
- Emerson Glassey
- Zhengan Zhang
- Christopher A. Voigt
