Cell culture articles from across Nature Portfolio

This protocol presents a versatile, easy-to-apply procedure for fabricating high-resolution cell-adhesive micropatterns, offering an affordable and scalable solution amenable to non-specialist cell biology teams.

This Protocol Extension details the standardized production of iPS cell-derived macrophages in an intermediate-scale benchtop bioreactor, providing a valuable tool for academic labs focused on human immune cells.

Neural tissue is a key signalling centre for fundic–antral patterning in development of gastric organoids featuring asymmetric PDX1 patterning.

We present a protocol to purify and expand human pluripotent stem cell-derived induced nephron progenitor cells (iNPCs) for long-term culture in a regular monolayer culture format and for the generation of nephron organoids.

A combination of organoid technology and genetic methods enables studies of human pathogens.

Immune–organoid systems will be the next generation of in vitro models.

New traceability and reporting standards aim to improve transparency in stem cell research.

Human cervical organoids mimic dynamics of cancer and sexually transmitted infections.