Abstract
Introduction
The work described here involved the use of a modified fundus camera to obtain sequential hyperspectral images of the retina in 14 normal volunteers and in 1 illustrative patient with a retinal vascular occlusion.
Methods
The paper describes analysis techniques, which allow oximetry within retinal vessels; these results are presented as retinal oximetry maps.
Results
Using spectral images, with wavelengths between 556 and 650 nm, the mean oxygen saturation (OS) value in temporal retinal arterioles in normal volunteers was 104.3 (±16.7), and in normal temporal retinal venules was 34.8 (±17.8). These values are comparable to those quoted in the literature, although, the venular saturations are slightly lower than those values found by other authors; explanations are offered for these differences.
Discussion
The described imaging and analysis techniques produce a clinically useful map of retinal oximetric values. The results from normal volunteers and from one illustrative patient are presented. Further developments, including the recent development of a ‘snapshot’ spectral camera, promises enhanced non-invasive retinal vessel oximetry mapping.
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Acknowledgements
This study was supported by Eye Therapy Trust; Technology Strategy Board (CHBT/007/00028). This paper presents independent research awarded under New and Emerging Applications of Technology (NEAT), part of the i4i Invention for Innovation programme (NEAT K034) of the National Institute for Health Research (NIHR). The views expressed in this publication are those of the author(s) and not necessarily those of the NHS, the NIHR, or the Department of Health. This study was approved by the Gloucestershire Research Ethics Committee (COREC reference: 06/Q2005/131) and all procedures were carried out in accordance with the tenets of the Declaration of Helsinki.
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AR Harvey owns a patent to a related spectral imaging device, and rest of the authors declare no conflict of interest.
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Mordant, D., Al-Abboud, I., Muyo, G. et al. Spectral imaging of the retina. Eye 25, 309–320 (2011). https://doi.org/10.1038/eye.2010.222
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DOI: https://doi.org/10.1038/eye.2010.222
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