Computational processing allows sub–diffraction-limit resolution imaging with a standard wide-field microscope.
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References
Wang, G. & Smith, S.J. Sub-diffraction limit localization of proteins in volumetric space using Bayesian restoration of fluorescence images from ultrathin specimens. PLoS Comput. Biol. 8, e1002671 (2012).
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Eisenstein, M. Attention to detail. Nat Methods 9, 1049 (2012). https://doi.org/10.1038/nmeth.2239
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DOI: https://doi.org/10.1038/nmeth.2239