Supplementary Figure 6: High-resolution cell image.
MCF-7 cells were cultured with 100 nM aptamer-FAM/GO-nS for 6 h with recommended media at 37 °C. Picture A and B were captured from two parallel experiments under the same operation conditions, respectively.
(Fluorescence imaging was carried out on an intensified charge-coupled device(ICCD)-based real-time fluorescence microscopy system, which was composed mainly of a TE2000 inverted microscope (Nikon, Japan) with a Nikon 100× N.A. 1.3 oil objective, an I-PentaMAX Gen IV ICCD camera (Roper Scientific, USA), and an xenon arc lamp in the Lambda DG-4 Wavelength Switcher (Sutter Instrument, USA). Selective excitation was produced through a 484 ± 7.5 nm band-pass filter. Fluorescence was monitored and quantitatively analyzed with the MetaMorph software (Universal Imaging Co., USA). Excitation light was adequately attenuated. Photo bleaching and cell damage were minimized by auto-synchronizing the exciting shutter and the exposure shutter, i.e. cells were exposed to light only during exposure but not at the interval. This ICCD fluorescence micro-imaging system possessed the advantages of high-performance thermoelectric cooling function to reduce noise, and a gain high enough to detect extremely low light.)
