Extended Data Fig. 2: Steps for vascular permeability measurements via confocal microscopy.
From: Engineered human blood–brain barrier microfluidic model for vascular permeability analyses
(a) Representative images of perfused plasma IgG (green) at times 0 and 12 min, and increasing matrix intensity over time; the single data points represent the same single pixels in the matrix imaged over time. Scale bar, 100 μm. (b) Permeability of plasma IgG in the BBB MVNs over time. At short times (<12 min), variability in the measurement derives from low signal-to-noise ratio. At long times (>20 min), higher permeability values derive from progressive perfusate diffusion from the side channels. (c) Comparison in 40 kDa dextran permeability measured in the micro and macro devices; n = 3 device repeats, each the average of 3 regions of interest (ROIs). The higher permeability in the micro device derives from increased perfusate diffusion from the side channels over the same time.
