Extended Data Fig. 3: Steps for vascular permeability measurements via fluid sampling.
From: Engineered human blood–brain barrier microfluidic model for vascular permeability analyses
(a) Example vascular and matrix intensities measured for IgG as a function of depth imaged within the device. Both intensities are normalized to the vascular intensity near the bottom glass. They decrease with depth as a result of light scattering, falling within the background intensity range (shaded area) in the case of the matrix intensity. (b) Example microscope z-drift measured during a typical experiment. (c) Permeability of plasma IgG as a function of imaged region of interest (ROI) size. Average and standard deviation between 3 devices are reported. For ROIs smaller than 600 µm by side, the varying permeability values are an artifact due to incomplete capture of the BBB MVN average morphology.
