Extended Data Fig. 5: Immunofluorescence staining for various proteins of interest in the BBB MVNs with iPS-ECs (steps 52-59 in the protocol). | Nature Protocols

Extended Data Fig. 5: Immunofluorescence staining for various proteins of interest in the BBB MVNs with iPS-ECs (steps 52-59 in the protocol).

From: Engineered human blood–brain barrier microfluidic model for vascular permeability analyses

Extended Data Fig. 5

(a) Staining of water channel protein (aquaporin 4) in ACs in the BBB MVNs. (b) Staining of glycocalyx protein (hyaluronic acid) in the BBB MVNs. (c) Staining of tight junction protein (claudin-5) in the BBB MVNs. Scale bars are 100 µm for (a-b) and 50 µm for (c).

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