Extended Data Fig. 6: Immunofluorescence staining for various proteins of interest in the BBB MVNs with HBMECs (steps 52-59 in the protocol). | Nature Protocols

Extended Data Fig. 6: Immunofluorescence staining for various proteins of interest in the BBB MVNs with HBMECs (steps 52-59 in the protocol).

From: Engineered human blood–brain barrier microfluidic model for vascular permeability analyses

Extended Data Fig. 6

(a) Staining of PC marker (PDGFR-β) in the MVNs, along with F-actin to visualize interactions between HBMECs and PCs. (b) Staining of AC marker (GFAP) in the MVNs, along with F-actin to visualize interactions between HBMECs and ACs. (c) Staining of tight junction protein (ZO-1) in the BBB MVNs with HBMECs. (d) Staining of basement membrane proteins (collagen IV and laminin) in the BBB MVNs with HBMECs. Scale bars are 100 µm for (a, d) and 50 µm for (b-c).

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