Extended Data Fig. 2: Equipment and assembly of imaging chamber for automated IBEX protocol. | Nature Protocols

Extended Data Fig. 2: Equipment and assembly of imaging chamber for automated IBEX protocol.

From: IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues

Extended Data Fig. 2

a, Tissues are sectioned onto coated 22 × 22 mm square coverslips and assembled into the RC-21B Large Closed Bath Imaging Chamber. b,c, Top view of imaging chamber placed into PM-2 Platform for Series 20 chambers without (b) and with (c) magnetic platform clamp. d, Equipment footprint of automated IBEX setup; FLPG. e, Complete assembly of PM-2 Platform with RC-21B chamber onto SA-20PL Series 20 stage adapter. Fluid inlet and vacuum outlet highlight the fluid path. Heating electrodes are attached to the top and bottom of the platform using metal prongs that must be bent by user to allow placement into the stage. Temperature probe is inserted into small hole at top of platform to maintain 37 °C for the duration of the protocol.

Back to article page