Extended Data Fig. 4: Key steps for data stitching, renaming and compression. | Nature Protocols

Extended Data Fig. 4: Key steps for data stitching, renaming and compression.

From: Scalable tissue labeling and clearing of intact human organs

Extended Data Fig. 4

a, One example for correcting information in ‘Stitching Image Grid Sequence’; a1 highlights the replacement of red letters in Step 42 about ‘file names’. b, The key information of ‘TileConfiguration’ txt file; b1 highlights the responding channel number that should be corrected and saved in the respective separate folder, and b2 highlights that the starting Z number should be ‘0000’ for all ‘TileConfiguration’ files. c, The information of ‘Stitching.py’. d, One example for settings of ‘Multi-Rename Tool. e, One example of compressing tiff files with ‘LZW TIFF’. Panel a produced with software from ref. 50, Oxford University Press.

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