Extended Data Fig. 1: Human brain clearing by perfusion system.
From: Scalable tissue labeling and clearing of intact human organs
a, The dissection of human brain with CL and CR (left and right carotids, respectively), VL and VR (left and right vertebral arteries, respectively). b, The human brain after dissection and fixation. c, The setup to clear a human brain by perfusion system under the fume hood. The human brain was put in a glass container and connected with input and output tubing controlled by a peristaltic pump. There are four channels controlling the four connecting tubes as indicated in c (i). Two output tubes are connected to the carotid arteries as shown in c (ii), and two output tubes are connected to the vertebral arteries as shown in c (iii). The input tubes are protected with gauze to avoid solid impurities entering and blocking the tubing (see c (iv)). c (v) shows the decolorization effects of CHAPS/NMDEA that manifest as the blooming of dark-green color from the organ. d, The glass container was sealed with several layers of plastic wrap to prevent evaporation of the running solutions, especially EtOH, DCM and MeOH. e, Photo of human brain after SHANEL pretreatment and before clearing. Remaining blood in the vessels (b) was decolorized.
