Extended Data Fig. 2: Passively stained conjugated antibody in centimeter-size human kidney and lung tissue. | Nature Protocols

Extended Data Fig. 2: Passively stained conjugated antibody in centimeter-size human kidney and lung tissue.

From: Scalable tissue labeling and clearing of intact human organs

Extended Data Fig. 2

a,b, Collagen IV, catalase and cytokeratin 19 antibody staining (green) in human kidney (a) and lung (b). Top panel in a and b shows immunofluorescent staining images obtained by confocal microscopy to verify antibody compatibility with SHANEL. Bottom panel shows 3D reconstruction of tissue with antibody labeling in Imaris. Scale bars, 20 µm and 500 µm, respectively.

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