Extended Data Fig. 5: LiGA binding to purified Siglec-6 and Siglec-6+ CHO cells.
From: Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)
a) Visual representation of the glycans in the LiGA. b) The binding pattern of LiGA on Siglec-6 expressing CHO cells showed no observable binding pattern, n = 5 test and n = 4 control samples. c) Binding of LiGA-100 purified Siglec 6 coated in a well, n = 5 test and n = 11 control samples. d) Purified Siglec-6 R122A mutant binding with LiGA-100. e) Differential enrichment of Siglec-6 compared to R122A mutant, n = 6 test and n = 11 control samples. The AzOH enrichment shows non-specific interaction among LiGA-100. All respective n values are independent biological replicates. In b–e, the FC was calculated by Bioconductor edgeR DE analysis using the negative binomial model, TMM normalization, and BH correction for FDR. Error bars represent s.d. propagated from the variance of the TMM-normalized sequencing data.
