Extended Data Fig. 1: Describes the workflow for generating the encoding glycan using SDB M13 phage.
From: Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)
a) The M13KE SDB SVEK library is a combination of two degenerate codon regions in the phage DNA: SB1 and SB2. The combination of SB1 and SB2 yields a total of 1.3 × 1010 DNA possible barcoded phages that are phenotypically identical. b) The M13KE SDB SVEK library was plated at ~100 plaques per plate. Each plaque (clone) was isolated, individually amplified, and purified with Triton X-100 and PEG to remove lipopolysaccharides. c) Schematic showing unmodified phage, DBCO-modified phage, and phage with azido-glycan. d) Workflow for modification of clonal phage with a distinct barcode. First, the phage is reacted with DBCO-NHS and verified by MALDI-TOF. Azide glycan is ligated with the DBCO on the phage. e) Typical MALDI-TOF spectra of unmodified phage, phage modified with DBCO, and after cycloaddition of azide glycan.
