Extended Data Fig. 3: LiGA profiling of hSiglec-9 on the surface of U937 and CHO cells.
From: Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)
a) Visual representation of the glycans in the LiGA. b) Siglec-9 expressing U937 cells with LiGA-100 enriched broad range of sialylated glycans, n = 5 test and n = 5 control samples. c) Siglec-9 expressing CHO cells showed a higher level of enrichment of the sialylated glycans and minor preference for a2-6 linked and poly-sialylated glycans, n = 5 test and n = 10 control samples. All respective n values are independent biological replicates. In b,c, the FC was calculated by Bioconductor edgeR DE analysis using the negative binomial model, TMM normalization, and BH correction for FDR. Error bars represent s.d. propagated from the variance of the TMM-normalized sequencing data. Red bars indicate glycans with non-background responses in the CHO cells but not in the U937 cells.
